GENE518 EXPERIMENTAL TECHNIQUES IN IMMUNOLOGY

Course Code:2190518
METU Credit (Theoretical-Laboratory hours/week):3 (3.00 - 0.00)
ECTS Credit:8.0
Department:Molecular Biology and Genetics
Language of Instruction:English
Level of Study:Graduate
Course Coordinator:Assist.Prof.Dr BANU BAYYURT KOCABAŞ
Offered Semester:Fall and Spring Semesters.

Course Objectives

To learn basic methods used in molecular biology studies including immunology area and immune system relared techniques such as immunoblotting.


Course Content

Introduction to current protocols in immunology, principles of experimental design to study an immunology related problem, introduction to animal models of infectious, inflammatory and autoimmune disease, animal models of transplantation and tumor immunology. Isolation of lymphoid cells from human and murine tissues and blood, principles of flow cytometry and its applications, proliferation and cytotoxicity assays, tetramer staining methods, ELİSA, ELİSPOT and cytometric bead array and adoptive cell transfer are among the major techniques that will be covered.


Course Learning Outcomes

Students will have an information about how and where the methods can be used in their research area. Besides, they will learn how they can interpret results in scientific articles.


Program Outcomes Matrix

Level of Contribution
#Program Outcomes0123
1Explain the inner workings of the scientific process, discuss critically, and solve problems by applying appropriate statistical and computational methods; choose contemporary experimental and theoretical approaches in molecular biology and genetics.
2Communicate issues in both English and Turkish with high proficiency.
3Read, and critique scientific communications in molecular biology and molecular genetics literature with worldwide recognition.
4Identify and explain issues in molecular biology and genetics within the context of other disciplines.
5Readily apply innovative theoretical and technological challenges acting upon the need for lifelong learning.
6Produce data and interpret results as an individual, as well as a team player, and lead whenever necessary.
7Apply and defend the rules of scientific ethics within their careers and professions.

0: No Contribution 1: Little Contribution 2: Partial Contribution 3: Full Contribution