GENE367 GENERAL AND MOLECULAR GENETICS LAB.
| Course Code: | 2190367 |
| METU Credit (Theoretical-Laboratory hours/week): | 2 (0.00 - 4.00) |
| ECTS Credit: | 3.0 |
| Department: | Molecular Biology and Genetics |
| Language of Instruction: | English |
| Level of Study: | Undergraduate |
| Course Coordinator: | Prof.Dr. AYŞE ELİF ERSON BENSAN |
| Offered Semester: | Fall Semesters. |
Course Objectives
Get the students familiar with methods such as electrophoresis, Southern Blotting, PCR, DNA isolaton and staining .
To make students handle living organisms like bacteria and Drosphila for genetic experiments.
Course Content
A laboratory course emphasizing the principles of genetics. Experiments include the application of transmission and population genetics using Drosophila as the model organism, such as segregation and independent assortment, sex linkage, linkage analysis and gene mapping, epistatic interactions, and gene frequency changes in populations. Polytene chromosomes and allozyme electrophoresis are also done with Drosophila. Chromosomal DNA, RNA and plasmid isolation techniques, UV induced mutations, genetic manipulation by recombinant DNA techniques and PCR are covered. The course has been designed for students of Molecular Biology and Genetics Program.
Course Learning Outcomes
Segregation and independent assortment analysis in Drosophila
Sex linkage, linkage analysis
Gene mapping
Epistatic interactions
Gene frequency changes in Drosophila populations
Polytene chromosomes
Allozyme electrophoresis
Chromosomal DNA, RNA and plasmid isolation in bacteria
UV induced mutations
Recombinant DNA techniques
PCR technique