CHE517 METABOLIC ENGINEERING
| Course Code: | 5630517 |
| METU Credit (Theoretical-Laboratory hours/week): | 3 (3.00 - 0.00) |
| ECTS Credit: | 8.0 |
| Department: | Chemical Engineering |
| Language of Instruction: | English |
| Level of Study: | Graduate |
| Course Coordinator: | Prof.Dr. PINAR ÇALIK |
| Offered Semester: | Fall Semesters. |
Course Objectives
Microorganisms as Microbioreactors: Industrially Important Microorganisms. Influence of bioreactor operation conditions on the bioreaction networks and regulation of metabolic pathways: fermentation physiology. Principles of metabolic engineering: Determination of the metabolic bottlenecks. Metabolic control analysis. Thermodynamic analysis of cellular pathways. Pathway design.
Course Content
Microorganism as a microbioreactors; Industrially important microorganisms. Influence of bioreactor operation conditions on the bioreaction networks and regulation of metabolic pathways: fermentation physiology. Principles of metabolic engineering: Determination of the metabolic bottlenecks. Metabolic control analysis. Thermodynamic analysis of cellular pathways. Pathway design.
Course Learning Outcomes
By the end of the course, the students will be able to:
- Define the main concepts of cellular metabolism
- Conduct the metabolic flux analysis
- Design promoters
- Recognize and apply genetic and biochemical tools for metabolic pathway design
- Design strategies to overcome rate-limiting steps and decrease by-product formation
Program Outcomes Matrix
| Contribution | |||||
| # | Program Outcomes | No | Yes | ||
| 1 | Acquire knowledge in depth and breadth via scientific research in their field; evaluate, interpret and apply this knowledge. | ✔ | |||
| 2 | Are thoroughly informed about current techniques and methods of engineering, and their limitations. | ✔ | |||
| 3 | Complement and apply uncertain, limited or incomplete knowledge using scientific methods; are capable of integrating knowledge from different disciplines. | ✔ | |||
| 4 | Are aware of the new and developing applications of their profession; can study and learn about these applications when necessary. | ✔ | |||
| 5 | Can define and formulate problems relevant to their field, develop solutions to solve these problems and employ innovative methods for these solutions. | ✔ | |||
| 6 | Develop new and/or original ideas and methods; design complex processes and develop innovative/alternative solutions in design. | ✔ | |||
| 7 | Design and apply theoretical, experimental and model-based research; analyze and resolve complex problems that arise during this process. | ✔ | |||
| 8 | Can effectively function within intra- and interdisciplinary teams, can lead such teams and formulate solution approaches under complex situations; can work independently and assume responsibility. | ✔ | |||
| 9 | Can communicate verbally or in written form in a non-native language, at least at level B2 of the European Language Portfolio. | ✔ | |||
| 10 | Can communicate the progress and results of their studies systematically and clearly in oral or written form, in national or international forums related to their area or others. | ✔ | |||
| 11 | Are informed and aware of the limitations of social, environmental, health and safety-related and legal dimensions on engineering applications. | ✔ | |||
| 12 | Uphold social, scientific and ethical values in acquisition, interpretation and communication of data and in all activities related to their profession. | ✔ | |||