CHE517 METABOLIC ENGINEERING

Course Code:5630517
METU Credit (Theoretical-Laboratory hours/week):3 (3.00 - 0.00)
ECTS Credit:8.0
Department:Chemical Engineering
Language of Instruction:English
Level of Study:Graduate
Course Coordinator:Prof.Dr. PINAR ÇALIK
Offered Semester:Fall Semesters.

Course Objectives

Microorganisms as Microbioreactors: Industrially Important Microorganisms. Influence of bioreactor operation conditions on the bioreaction networks and regulation of metabolic pathways: fermentation physiology. Principles of metabolic engineering: Determination of the metabolic bottlenecks. Metabolic control analysis. Thermodynamic analysis of cellular pathways. Pathway design. 


Course Content

Microorganism as a microbioreactors; Industrially important microorganisms. Influence of bioreactor operation conditions on the bioreaction networks and regulation of metabolic pathways: fermentation physiology. Principles of metabolic engineering: Determination of the metabolic bottlenecks. Metabolic control analysis. Thermodynamic analysis of cellular pathways. Pathway design.


Course Learning Outcomes

 By the end of the course, the students will be able to:

  • Define the main concepts of cellular metabolism 
  • Conduct the metabolic flux analysis
  • Design promoters
  • Recognize and apply genetic and biochemical tools for metabolic pathway design
  • Design strategies to overcome rate-limiting steps and decrease by-product formation

Program Outcomes Matrix

Contribution
#Program OutcomesNoYes
1Acquire knowledge in depth and breadth via scientific research in their field; evaluate, interpret and apply this knowledge.
2Are thoroughly informed about current techniques and methods of engineering, and their limitations.
3Complement and apply uncertain, limited or incomplete knowledge using scientific methods; are capable of integrating knowledge from different disciplines.
4Are aware of the new and developing applications of their profession; can study and learn about these applications when necessary.
5Can define and formulate problems relevant to their field, develop solutions to solve these problems and employ innovative methods for these solutions.
6Develop new and/or original ideas and methods; design complex processes and develop innovative/alternative solutions in design.
7Design and apply theoretical, experimental and model-based research; analyze and resolve complex problems that arise during this process.
8Can effectively function within intra- and interdisciplinary teams, can lead such teams and formulate solution approaches under complex situations; can work independently and assume responsibility.
9Can communicate verbally or in written form in a non-native language, at least at level B2 of the European Language Portfolio.
10Can communicate the progress and results of their studies systematically and clearly in oral or written form, in national or international forums related to their area or others.
11Are informed and aware of the limitations of social, environmental, health and safety-related and legal dimensions on engineering applications.
12Uphold social, scientific and ethical values in acquisition, interpretation and communication of data and in all activities related to their profession.