BIOL545 PRINCIPLES OF FLUORESCENCE SPECTROSCOPY AND MICROSCOPY
| Course Code: | 2380545 |
| METU Credit (Theoretical-Laboratory hours/week): | 3 (3.00 - 0.00) |
| ECTS Credit: | 8.0 |
| Department: | Biology |
| Language of Instruction: | English |
| Level of Study: | Graduate |
| Course Coordinator: | Prof.Dr. ÇAĞDAŞ DEVRİM SON |
| Offered Semester: | Spring Semesters. |
Course Objectives
Throughout the course, developments in instrumentation and fluorescence labels will be introduced. Applications of the new fluorescence dyes and proteins to biological samples will be discussed. Case studies of new methods such as single-molecule detection in living cells, fluorescence energy transfer techniques to identify protein-protein interactions, and fluorescent biosensors will be introduced.
Course Content
Upon completion of the course students will gain insight on developments in the instrumentation and use of fluorescence labels. They will be familiarized with the applications of the new fluorescence dyes and proteins to biological samples. Review of case studies of new methods such as single molecule detection in living cells, fluorescence energy transfer techniques to identify protein-protein interactions and fluorescent biosensors will help students excel in this developing field.
Course Learning Outcomes
Types of spectroscopy and microscopy will be learned. Applications of fluorescent dyes and their properties, which types of microscopes are used for what purpose will be explained. Students who complete the course should be able to critically read and understand current literature on imagining and spectroscopy.
Program Outcomes Matrix
| Level of Contribution | |||||
| # | Program Outcomes | 0 | 1 | 2 | 3 |
| 1 | Explain the inner workings of the scientific process, discuss critically, and solve problems by applying appropriate statistical and computational methods; choose experimental and theoretical approaches in biology. | ✔ | |||
| 2 | Communicate issues in both English and Turkish with high proficiency. | ✔ | |||
| 3 | Read, and critique scientific communications in biology literature with worldwide recognition. | ✔ | |||
| 4 | Identify and explain issues in biological sciences within the context of other disciplines. | ✔ | |||
| 5 | Readily apply innovative theoretical and technological challenges acting upon the need for lifelong learning. | ✔ | |||
| 6 | Produce data and interpret results as an individual, as well as a team player, and lead whenever necessary. | ✔ | |||
| 7 | Apply and defend the rules of scientific ethics within their careers and professions. | ✔ | |||
0: No Contribution 1: Little Contribution 2: Partial Contribution 3: Full Contribution