BIOL311 INSTRUMENTAL METHODS IN BIOLOGY
| Course Code: | 2380311 |
| METU Credit (Theoretical-Laboratory hours/week): | 3 (2.00 - 2.00) |
| ECTS Credit: | 6.0 |
| Department: | Biology |
| Language of Instruction: | English |
| Level of Study: | Undergraduate |
| Course Coordinator: | Prof.Dr. ORHAN ADALI |
| Offered Semester: | Fall Semesters. |
Course Objectives
This course consists of a theoretical and a practical laboratory part.
In the theoretical part, a number of techniques used for isolation, purification and characterization of biological molecules such as proteins are taught.
In the practical part, 12 experiments prepared by assistants are demonstrated to teach the techniques of analysis and isolation of biological molecules. These include buffer preparation and use of pH meter, homogenization, centrifugation, chromatography, electrophoresis and spectrophotometry.
Course Content
A comprehensive course on the principles and applications of techniques widely used by biologists. The techniques include; centrifuga tion, chromatography, spectrophotometry, elec trophoresis, and use of radioisotopes and microscopy.
Course Learning Outcomes
1. Describe the preparation of solutions at different concentration unit
2. Describe the preparation of buffers at desired pH.
3. List the techniques and appraoches to study biological and cellular processes at different organismal level.
4. Describe and conduct the techniques of tissue and cell homogenization and centrifugation.
5. Explain the types of chromatography and electrophoresis for isolation and purification of molecules.
6. Explain and conduct the spectrophotometry for analysis of molecules.
Program Outcomes Matrix
| Level of Contribution | |||||
| # | Program Outcomes | 0 | 1 | 2 | 3 |
| 1 | Perform basic laboratory and field techniques/approaches used in various branches of biology. | ✔ | |||
| 2 | Understand and apply principles of laboratory and field safety. | ✔ | |||
| 3 | Understand the inner workings of the scientific processes, think critically, and frame questions using experimental and theoretical approaches in biology. | ✔ | |||
| 4 | Proficiency in communicating issues in both English and Turkish. | ✔ | |||
| 5 | Read, and understand scientific communications from primary literature in biology. | ✔ | |||
| 6 | Identify and explain issues in biological sciences. | ✔ | |||
| 7 | Be ready to take new theoretical and technological challenges by recognizing the value and act upon the need for lifelong learning. | ✔ | |||
| 8 | Work as an individual, as well as a team member, and assume leadship when needed. | ✔ | |||
| 9 | Follow the rules of scientific ethics within their careers and professions. | ✔ | |||
0: No Contribution 1: Little Contribution 2: Partial Contribution 3: Full Contribution