BIOL311 INSTRUMENTAL METHODS IN BIOLOGY
| Course Code: | 2380311 |
| METU Credit (Theoretical-Laboratory hours/week): | 3 (2.00 - 2.00) |
| ECTS Credit: | 6.0 |
| Department: | Biology |
| Language of Instruction: | English |
| Level of Study: | Undergraduate |
| Course Coordinator: | Prof.Dr. ORHAN ADALI |
| Offered Semester: | Fall Semesters. |
Course Objectives
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Introduce biomolecules, cellular structures, and their roles in instrumental analysis.
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Develop skills in laboratory-related calculations, pH and buffer concepts.
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Explain principles and applications of key techniques in cell culture, separation, spectrophotometry, chromatography, electrophoresis, immunoassays, enzyme kinetics, and PCR.
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Engage in interactive, discussion- and analysis-based activities to evaluate protocols and experiments.
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Discuss the selection and optimization of reagents, instruments, and experimental conditions for specific biological applications.
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Evaluate research articles and video demonstrations to identify experimental design choices, data analysis approaches, and methodological limitations.
Course Content
A comprehensive course on the principles and applications of techniques widely used by biologists. The techniques include; centrifuga tion, chromatography, spectrophotometry, elec trophoresis, and use of radioisotopes and microscopy.
Course Learning Outcomes
1. Perform basic laboratory-related calculations, including molarity, dilutions, and pH/buffer preparations.
2. List the techniques and approaches to study biological and cellular processes.
3. Explain the principles and applications of biological techniques such as cell culture, separation, spectrophotometry, chromatography, electrophoresis, immunoassays, enzyme kinetics, and PCR.
4. Critically assess experimental data and protocols, reagents and instrumentation for specific applications, and discuss scientific findings effectively
Program Outcomes Matrix
| Level of Contribution | |||||
| # | Program Outcomes | 0 | 1 | 2 | 3 |
| 1 | Perform basic laboratory and field techniques/approaches used in various branches of biology. | ✔ | |||
| 2 | Understand and apply principles of laboratory and field safety. | ✔ | |||
| 3 | Understand the inner workings of the scientific processes, think critically, and frame questions using experimental and theoretical approaches in biology. | ✔ | |||
| 4 | Proficiency in communicating issues in both English and Turkish. | ✔ | |||
| 5 | Read, and understand scientific communications from primary literature in biology. | ✔ | |||
| 6 | Identify and explain issues in biological sciences. | ✔ | |||
| 7 | Be ready to take new theoretical and technological challenges by recognizing the value and act upon the need for lifelong learning. | ✔ | |||
| 8 | Work as an individual, as well as a team member, and assume leadship when needed. | ✔ | |||
| 9 | Follow the rules of scientific ethics within their careers and professions. | ✔ | |||
0: No Contribution 1: Little Contribution 2: Partial Contribution 3: Full Contribution